Natural and artificial mutants as valuable resources for functional genomics and molecular breeding

Natural and artificial mutants as valuable resources for functional genomics and molecular breeding

With the completion of rice genome sequencing, large collection of expression data and the great efforts in annotating rice genomes, the next challenge is to systematically assign functions to all predicted genes in the genome. The generations and collections of mutants at the genome-wide level form technological platform of functional genomics. In this study, we have reviewed currently employe...

Legume Genomics: From Genomic Resources to Molecular Breeding

With an explosive growth rate, especially in developing countries, the world population of 7.2 billion is expected to reach 9.6 billion by 2050. There is a need to produce about 70% more food to feed this predicted population. Legumes form important constituents of a vegetarian diet and are rich sources of dietary protein (Duranti and Gius, 1997). Legumes comprise the third largest family of fl...

Breeding and Genetics: Molecular Biology and Genomics

Preservation of bio-specimens for molecular biological applications traditionally involves freezing which increases laboratory and research project costs. Protection and stabilization of DNA at room temperature (RT) may eliminate the costs associated with freezer storage. However, there is a paucity of information describing the yield, purity, and integrity of DNA stored at RT. Objectives were ...

Tourism And Natural Resources

Potential of a mutant-based reverse genetic approach for functional genomics and molecular breeding in soybean

Mutant-based reverse genetics offers a powerful way to create novel mutant alleles at a selected locus. This approach makes it possible to directly identify plants that carry a specific modified gene from the nucleotide sequence data. Soybean [Glycine max (L.) Merr.] has a highly redundant paleopolyploid genome (approx. 1.1 Gb), which was completely sequenced in 2010. Using reverse genetics to ...